Coding
"G"

Part:BBa_K1080009:Design

Designed by: Macquarie University   Group: iGEM13_Macquarie_Australia   (2013-09-22)

ChlG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 386
    Illegal BglII site found at 875
    Illegal BamHI site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence

Amino acid sequence

MNQQATEEKS DTNSAARQML GMKGAALETD IWKIRVQLTK PVTWIPLIWG VACGAAASGH YQWNNPTQIA QLLTCMMMSG PFLTGYTQTI NDWYDREIDA INEPYRPIPS GRISERDVIV QIWVLLLGGI GLAYTLDQWA GHTTPVMLQL TIFGSFISYI YSAPPLKLKQ SGWAGNYALG SSYIALPWWA GQALFGTLTL DVMALTIAYS LAGLGIAIVN DFKSIEGDRQ MGLQSLPVAF GVDTAKWICV STIDVTQLGV AAYLAWGLHE ELYGAVLLAL ILPQIYFQYK YFLPDPIAND VKYQASAQPF LVFGLLTAGL ACGHHVNAVA AAASAAGAL

References and documentation are available. Please note the modified algorithm for extinction coefficient.

Number of amino acids: 339

Molecular weight: 36830.6

Theoretical pI: 5.30

Amino acid composition: Ala (A) 43 12.7% Arg (R) 7 2.1% Asn (N) 10 2.9% Asp (D) 14 4.1% Cys (C) 4 1.2% Gln (Q) 20 5.9% Glu (E) 9 2.7% Gly (G) 30 8.8% His (H) 5 1.5% Ile (I) 26 7.7% Leu (L) 40 11.8% Lys (K) 10 2.9% Met (M) 9 2.7% Phe (F) 10 2.9% Pro (P) 16 4.7% Ser (S) 18 5.3% Thr (T) 21 6.2% Trp (W) 12 3.5% Tyr (Y) 16 4.7% Val (V) 19 5.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% 

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23 Total number of positively charged residues (Arg + Lys): 17

Atomic composition:

Carbon C 1694 Hydrogen H 2600 Nitrogen N 422 Oxygen O 471 Sulfur S 13

Formula: C1694H2600N422O471S13 Total number of atoms: 5200

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 90090 Abs 0.1% (=1 g/l) 2.446, assuming all pairs of Cys residues form cystines


Ext. coefficient 89840 Abs 0.1% (=1 g/l) 2.439, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 

                            30 hours (mammalian reticulocytes, in vitro).
                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.91 This classifies the protein as stable.


Aliphatic index: 104.87

Grand average of hydropathicity (GRAVY): 0.327


Source

Chlamydomonas reinhardtii

References